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jacalin conjugated agarose beads  (Vector Laboratories)


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    Structured Review

    Vector Laboratories jacalin conjugated agarose beads
    Jacalin Conjugated Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jacalin+conjugated+agarose+beads/Agarose+Bound+Peanut+Agglutinin+(PNA)/pmc11788431-350-45-48
    Average 92 stars, based on 33 article reviews
    jacalin conjugated agarose beads - by Bioz Stars, 2026-10
    92/100 stars

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    Incubation:

    Article Title: Autoantibodies in endometriosis sera recognize a Thomsen-Friedenreich-like carbohydrate antigen.
    Article Snippet: Received 18 August 2000 Accepted 2 November 2000 Key words: autoantibodies, carbohydrates, endometriosis, sialic acid, Thomsen–Friedenreich antigen Autoantibody responses to endometrial antigens are a common feature of endometriosis.. Antibody responses to a number of serum and tissue antigens such as 2-Heremans Schmidt glycoprotein ( 2-HSG), transferrin, and carbonic anhydrase have been identified.. The nature of the epitopes recognized on these proteins has not been determined.

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: .. A673 or TC-71 whole-cell lysates (600 μg) were pre-treated with 100 U α2-3,6,8 neuraminidase (New England BioLabs, Cat# P0720S) in the presence or absence of 800 U O-glycosidase (New England BioLabs, Cat# P0733S) for 2 h at room temperature, and then incubated with PNA- or jacalin-conjugated agarose beads (Vector Laboratories, Cat# AL-1073 or Cat# AL-1153-10, respectively) overnight at 4 °C. ..

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.
    Article Snippet: .. A673 or TC-71 whole-cell lysates (600μg) were pre-treated with 100U α2-3,6,8 neuraminidase (New England BioLabs, Cat# P0720S) in the presence or absence of 800U O-glycosidase (New England BioLabs, Cat# P0733S) for 2 h at room temperature, and then incubated with PNA- or jacalin-conjugated agarose beads (Vector Laboratories, Cat# AL-1073 or Cat# AL-1153-10, respectively) overnight at 4 °C. ..

    Immunoprecipitation:

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation.
    Article Snippet: .. Lectin pull down and immunoprecipitation(IP) For lectin pull down assay, 1 mg total cell lysates were applied to Jacalin conjugated agarose beads (AL11153; Vector Laboratories) at 4°C overnight. .. For IP assay, total cell lysates (1 mg) were incubated with 10 μl anti-β1-integrin antibodies (ab24693; Abcam) and protein G sepharose beads (GE Heathcare, Chalfont, UK) at 4°C overnight.

    Pull Down Assay:

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation.
    Article Snippet: .. Lectin pull down and immunoprecipitation(IP) For lectin pull down assay, 1 mg total cell lysates were applied to Jacalin conjugated agarose beads (AL11153; Vector Laboratories) at 4°C overnight. .. For IP assay, total cell lysates (1 mg) were incubated with 10 μl anti-β1-integrin antibodies (ab24693; Abcam) and protein G sepharose beads (GE Heathcare, Chalfont, UK) at 4°C overnight.

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation
    Article Snippet: .. For lectin pull down assay, 1 mg total cell lysates were applied to Jacalin conjugated agarose beads (AL11153; Vector Laboratories) at 4°C overnight. .. For IP assay, total cell lysates (1 mg) were incubated with 10 μl anti-β1-integrin antibodies (ab24693; Abcam) and protein G sepharose beads (GE Heathcare, Chalfont, UK) at 4°C overnight.



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    jacalin conjugated agarose beads  (Vector Laboratories)
    92
    Vector Laboratories jacalin conjugated agarose beads
    Jacalin Conjugated Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jacalin+conjugated+agarose+beads/Agarose+Bound+Peanut+Agglutinin+(PNA)/pmc11788431-350-45-48
    Average 92 stars, based on 1 article reviews
    jacalin conjugated agarose beads - by Bioz Stars, 2026-10
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    jacalin-conjugated agarose beads  (Millipore)
    90
    Millipore jacalin-conjugated agarose beads
    Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight <t>with</t> <t>jacalin-conjugated</t> agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.
    Jacalin Conjugated Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jacalin+conjugated+agarose+beads/jacalin+agarose/pmc00135985-93-0-3
    Average 90 stars, based on 1 article reviews
    jacalin-conjugated agarose beads - by Bioz Stars, 2026-10
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    agarose conjugated jacalin beads  (Thermo Fisher)
    99
    Thermo Fisher agarose conjugated jacalin beads
    Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight <t>with</t> <t>jacalin-conjugated</t> agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.
    Agarose Conjugated Jacalin Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jacalin+conjugated+agarose+beads/Agarose/pm09788883-84-12-15
    Average 99 stars, based on 1 article reviews
    agarose conjugated jacalin beads - by Bioz Stars, 2026-10
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    Image Search Results


    Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight with jacalin-conjugated agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.

    Journal:

    Article Title: Distinct Glycoprotein O Complexes Arise in a Post-Golgi Compartment of Cytomegalovirus-Infected Cells

    doi: 10.1128/JVI.76.6.2890-2898.2002

    Figure Lengend Snippet: Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight with jacalin-conjugated agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.

    Article Snippet: Jacalin-conjugated agarose beads (Sigma) were equilibrated in radioimmunoprecipitation assay (RIPA) medium-0.5% bovine serum albumin (BSA; Sigma) prior to use.

    Techniques: Incubation, SDS Page, Western Blot